Detection of miRNAs by small RNA gel blot hybridisation. (A) Physcomitrella miRNAs expressed in protonema (P) and gametophore (G) tissue. (B) Physcomitrella miRNAs with a tissue-specific expression pattern. (C) Tissue-specific processing of miRNA precursors. The mature miRNAs were detected in RNA derived from protonema tissue, longer incompletely processed precursor transcripts were present in RNA from gametophores. The lowermost panel shows two representative ethidium bromide stained gels to indicate equal loading of the RNAs.