Table 3 Analysis of variance with respect to frequency of tissue-culture induced (epi)mutations for explant and donor.
From: Quantification of the tissue-culture induced variation in barley (Hordeum vulgare L.)
| Â | df | SS | MS | F-ratio | p |
|---|---|---|---|---|---|
Explant | 1 | 0.064 | 0.064 | 1.32 | 0.26 ns |
Donor | 4 | 3.835 | 0.959 | 19.95 | < .0001 |
Explant × Donor | 4 | 0.042 | 0.010 | 0.22 | 0.93 ns |
Variation class | 3 | 0.068 | 0.023 | 0.15 | 0.93 ns |
donor | mean | variation class | mean | Â | Â |
4DH | 1.77a | demethylation | 1.33a | Â | Â |
8DH | 1.38b | de novo methylation | 1.27a | Â | Â |
9DH | 1.24b | sequence variation | 1.24a | Â | Â |
5DH | 1.09bc | complex variation | 1.22a | Â | Â |
16DH | 0.84c | Â | Â | Â | Â |