Actin disruption accelerates loss of polar endomembrane labeling. At 6.5 h AF, zygotes on coverslips in Petri dishes were labeled for 30 min followed by 3 × 5 min rinses in ASW to wash out FM4-64. Zygotes were immediately scored for initial endomembrane polarity. Either 0.06% DMSO or 30 nM Lat B was then added to a dish and endomembrane polarity was again scored 1 and 2 h later on separate coverslips. At the time indicated by the arrow (2 h after FM4-64 washout), the media were spiked with FM4-64. Y axis indicates percent of zygotes with polar endomembrane distribution. Over 100 zygotes were scored for each point and the experiment was repeated three times. Bars are standard errors.