Figure 4

EMSA showing enhancement of binding activity of Nonabokra NE with ³²P-1X ABRE-DNA after addition of the S₈₀ fraction. A. Enhancement of the binding intensity by pre-incubation of control NE from Nonabokra with S₈₀ fraction prepared from Nonabokra (200 mM NaCl for 16 hrs; lane 2). In lane 1, no S₈₀ fraction was added. B. EMSA showing enhancement of complex formation after addition of ATP (lane 1). Partial inhibition of binding was obtained with heparin (a potent inhibitor of casein kinase II, lane 2), whereas no inhibition was obtained with phenyl arsine oxide (an indirect inhibitor of MAP kinase, lane 3) or R24571 (a potent inhibitor of CDPK, lane 4).