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Volume 5 Supplement 1

Cell Biology of Nitric Oxide and Cell Death in Plants

High-scale analysis of pathogenicity determinants of Potato mop-top virus

BMC Plant Biology volume 5, Article number: S39 (2005) Cite this article

Potato mop-top virus (PMTV) is the type member of the genus Pomovirus characterized by a tripartite, single-stranded, positive-sense RNA genome. However, PMTV differs from the other pomoviruses by having an open reading frame (ORF) for a 8-kDa cysteine-rich protein (CRP) on RNA 3, downstream the triple gene block. Site-directed mutagenesis of infectious cDNA clones of PMTV showed that the CRP ORF is not needed for the systemic infection in Nicotiana benthamiana and several other hosts, but it has an effect on the symptom development of PMTV. CRP of PMTV was not capable of RNA silencing suppression in agroinfiltration tests. However, the expression of CRP using vectors based on Potato virus X (PVX) or Tobacco mosaic virus (TMV) resulted in necrotic symptoms on N. benthamiana and tobacco plants, respectively. Sequencing of PMTV isolates obtained from the field revealed significant sequence variability of the CRP. These data suggest that the functions of CRP may be expressed only in certain hosts or at certain phases of the PMTV infection cycle.

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Authors and Affiliations

  1. Natural Sciences Center of A.M. Prokhorov General Physics Institute, Russian Academy of Sciences, Vavilov Str, 38, Bld. L-2, Moscow, 119991, Russia

    Andrey Zamyatnin

  2. Department of Plant Biology and Forest Genetics, Genetics Centre, SLU, Uppsala, S-750 07, Sweden

    Andrey Zamyatnin, Anna Germundsson, Maria Sandgren, Jari Valkonen & Eugene Savenkov

  3. A. N. Belozersky Institute of Physico-Chemical Biology and Department of Virology, Moscow State University, Moscow, 119899, Russia

    Natasha Yelina, Nina Lukhovitskaya, Andrey Solovyev & Sergey Morozov

Authors
  1. Andrey Zamyatnin
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  2. Natasha Yelina
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  3. Nina Lukhovitskaya
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  4. Andrey Solovyev
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  5. Anna Germundsson
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  6. Maria Sandgren
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  7. Sergey Morozov
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  8. Jari Valkonen
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  9. Eugene Savenkov
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Correspondence to Andrey Zamyatnin.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Zamyatnin, A., Yelina, N., Lukhovitskaya, N. et al. High-scale analysis of pathogenicity determinants of Potato mop-top virus. BMC Plant Biol 5 (Suppl 1), S39 (2005). https://doi.org/10.1186/1471-2229-5-S1-S39

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  • DOI: https://doi.org/10.1186/1471-2229-5-S1-S39

Keywords

  • Tobacco Mosaic Virus
  • Potato Virus
  • Symptom Development
  • Gene Block
  • Sequence Variability

BMC Plant Biology

ISSN: 1471-2229

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