Figure 3

AML loci and insertion alleles. The position of the insertion in each locus is indicated by a triangle below the locus picture. The wild-type loci were described previously (17). The thin central line indicates genomic DNA. Solid blue blocks spanning the central line indicate coding exons. Solid blue blocks below the line indicate untranslated regions. T-DNA insertion left borders are indicated with a triangle below the locus. Binding sites for primers used to genotype the wild-type allele of each locus are indicated with arrows. Binding sites for primers used to ascertain the effect of homozygosity at the mutant allele are indicated with triangles tagged by a single hash line if the PCR product spans the insertion site, or a doublet hash line if the PCR product is from a region downstream of the insertion site.