Figure 4

Purification of recombinant AtGA3OX4 shown by SDS-PAGE on a 8–15% gradient gel with Coomassie Blue staining.(A) Lanes: 1, soluble cell fraction; 2, TRX-AtGA3OX4 after IMAC chromatography; 3, TRX-AtGA3OX4 after thrombin digest; 4, concentrated TRX-AtGA3OX4 (10 mg/ mL); 5, Bio Rad molecular weight standards, (from top) 115, 93, 49.8, 35.8, 29.2, 21.3 and 6.4 kDa.(B) Lanes: 1, TRX-AtGA3OX4 after thrombin digest; 2, AtGA3OX4 (1.5 mg/mL, maximal concentration achieved) after gel filtration on Superdex 75; 3, TRX-AtGA3OX4 (10 mg/mL) after gel filtration on Superdex 200. The molecular weights expected for TRX-AtGA3OX4, AtGA3OX4 and TRX are 54.1, 39.1 and 15 kDa, respectively. The arrows indicate the position of AtGA3OX4.