From: Development of ESTs from chickpea roots and their use in diversity analysis of the Cicergenus
Name | Profile | Temperature°C | Time min. sec | Number of cycles |
---|---|---|---|---|
65-60 | Denaturation | 95 | 3.00 | 1 |
 | Touch down | 94 | 0.20 | 5 |
 |  | 65-60 | 0.20 |  |
 |  | 72 | 0.30 |  |
 | Normal | 94 | 0.20 | 30 |
 |  | 59 | 0.20 |  |
 |  | 72 | 0.30 |  |
 | Extension | 72 | 20.0 | 1 |
60-55 | Denaturation | 95 | 3.00 | 1 |
 | Touch down | 94 | 0.20 | 5 |
 |  | 60-55 | 0.20 |  |
 |  | 72 | 0.30 |  |
 | Normal | 94 | 0.20 | 30 |
 |  | 56 | 0.20 |  |
 |  | 72 | 0.30 |  |
 | Extension | 72 | 20.0 | 1 |
55-45 | Denaturation | 95 | 3.00 | 1 |
 | Touch down | 94 | 0.20 | 10 |
 |  | 55-45 | 0.20 |  |
 |  | 72 | 0.30 |  |
 | Normal | 94 | 0.20 | 30 |
 |  | 48 | 0.20 |  |
 |  | 72 | 0.30 |  |
 | Extension | 72 | 20.0 | 1 |