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Table 4 PCR amplification profiles used for optimization of PCR conditions for each marker.

From: Development of ESTs from chickpea roots and their use in diversity analysis of the Cicergenus

Name Profile Temperature°C Time min. sec Number of cycles
65-60 Denaturation 95 3.00 1
  Touch down 94 0.20 5
   65-60 0.20  
   72 0.30  
  Normal 94 0.20 30
   59 0.20  
   72 0.30  
  Extension 72 20.0 1
60-55 Denaturation 95 3.00 1
  Touch down 94 0.20 5
   60-55 0.20  
   72 0.30  
  Normal 94 0.20 30
   56 0.20  
   72 0.30  
  Extension 72 20.0 1
55-45 Denaturation 95 3.00 1
  Touch down 94 0.20 10
   55-45 0.20  
   72 0.30  
  Normal 94 0.20 30
   48 0.20  
   72 0.30  
  Extension 72 20.0 1
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BMC Plant Biology

ISSN: 1471-2229

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