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Figure 2 | BMC Plant Biology

Figure 2

From: A systemic gene silencing method suitable for high throughput, reverse genetic analyses of gene function in fern gametophytes

Figure 2

Gametophyte phenotypes after bombardment with 35S:irintCrChlI. (A) Scanning electron microscopy of 6 day old hermaphrodite initiating a lateral meristem. (B) Non-bombarded 15 day-old hermaphrodite showing meristem notch (mn), archegonia (ar) and antheridia (an). (C) A 15d-old hermaphrodite 8d after bombardment with a large colorless sector including the meristem and gametangia. (D) Bombarded colorless gametophyte stained for GUS activity. (E)-(H) Phenotypes of self-fertilized colorless gametophytes ~3 weeks after bombardment. Embryos are indicated by arrows.

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