Table 1 Arabidopsis lines used in this study. All lines contain mutations that primarily affect the composition of chloroplast membranes involved in photosynthesis.
Line | Biochemical defect | Primary affect on membrane composition | Reference |
|---|---|---|---|
fad5 | 16:0 to 16:1 desaturation on sn-2 of all chloroplast galactolipids | Elevated 16:0; no 16:3 in chloroplast membranes | [32] |
fad6 | 16:1 to 16:2 and 18:1 to 18:2 desaturation on sn-1 and sn-2 of all chloroplast lipids | Elevated 16:1 and 18:1; no 16:3, diminished 18:2, 18:3 | [33] |
fad7 fad8 | 16:2 to 16:3 and 18:2 to 18:3 desaturation on sn-1 and sn-2 of all chloroplast lipids | Elevated 16:2 and 18:2; diminished 16:3 and 18:3 | [35] |
act1 | Plastid glycerol-3-phosphate acyltransferase (GPAT) activity; first step in the prokaryotic pathway | Slightly elevated 18:1; diminished levels of 16-C fatty acids (prokaryotic pathway) | [34] |
act1 fad6 | Double mutant containing both the act1 and fad6 mutations | Slightly elevated levels of 18:1 (than act1 alone); no elevation of 16:1 (as in fad6); prokaryotic pathway blocked | This work |
act1 ++ | Plastid GPAT overexpressor | Similar to wild type. Slightly elevated levels of 16:3, reflecting slightly enhanced flux through prokaryotic pathway | This work |