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Table 1 List of cloning primers

From: Fusion to GFP blocks intercellular trafficking of the sucrose transporter SUT1 leading to accumulation in companion cells

Primer Name Sequencea
KpnI-mGFP5 TCG GGTACC ATG AGT AAA GGA GAA GAA CTT TTC
SacI/EagI-mGFP5 GTGT CGGCCGGAGCTC TTA TTT GTA TAG TTC ATC CAT GCC
KpnI-GUS CTCG GGTACC ATG TTA CTT CCT GTA GAA ACC
SacI/EagI-GUS GTGT CGGCCGGAGCTC TCA TTG TTT GCC TCC CTG C
KpnI-LeSUT1 GAA GGTACC CAA ATG GAG AAT GGT ACA AAA GGG
SacI/EagI-LeSUT1 CGTCTT CGGCCGGAGCTC TTA ATG GAA ACC GCC CAT GG
EagI-3'-UTR TTC CGGCCG AAA AAA TTA CAA AAG ACG AGG AAG
SacI-3'-UTR TACC GAGCTCCTAGG CGA GGT CGA CGG TAT CG
NotI-LeSUT1 CGTCTT GCGGCCGC TTA ATG GAA ACC GCC CAT GG
NotI-GFP TCG GCGGCCGC TTA TTT GTA TAG TTC ATC CAT GCC
  1. a Restriction sites are underlined