BFL1 transcript analysis by RT-PCR. Semi-quantitative detection of BFL1 transcripts in total RNA samples isolated from wild-type leaf (lane 1) and developing panicle (lane 3) and mutant leaf (lane 2) and developing panicle (lane 4), and same samples without DNase treatment (lanes 5–8) amplified using BFL1 gene-specific primers. Lanes 10–13 are same samples amplified using rice RSs1 gene specific primers (binding to two exons flanking an intron) to serve as internal controls for RNA quantity and DNA contamination. Semi-quantitative detection of BFL1 transcripts along with internal control (RSs1) in total RNA samples isolated from wild-type (lane 14), bfl1 mutant (lane 15) and revertant (lane 16) panicles. Lanes 9 and 17 are the molecular wt. markers.