Genomic location of BFL1 and Ds insertion in bfl1 mutant. Schematic representation of the positions of the BFL1 gene and the Ds insertion in bfl1 mutant in the Rice genomic PAC clone P0625E02 (GenBank Acc. No.AP004570). The orientation of the BFL1 gene (P0625E02.25) is indicated by arrow. Binding sites of primers Ds5-112- (P1), Ds3-6587+ (P2), LW1125_Rev (P3), LW1125_For (P4) used in PCR1, PCR2 and PCR3, and OsBfl1_Rev2 (P5) and OsBfl1_For (P6) used in RT-PCR (see Table 2 for details) are indicated. Solid line represents gus probe used for Southern blot hybridization analysis. HindIII restriction sites are indicated (H).