Figure 6

RNA chaperone activity of the CFM4 protein. (A) Complementation ability of CFM4 in E. coli BX04 mutant cells during cold shock. The diluted cultures of E. coli cells expressing CFM4, CspA (positive control), or pINIII vector (negative control) were spotted on LB-agar plates, and the cells were incubated at either 37°C or 20°C. (B) DNA-melting ability of CFM4. Fluorescence of the molecular beacons was measured after adding the recombinant GST-CFM4, GST-CspA (positive control), or GST (negative control) proteins. (C) Transcription anti-termination activity of CFM4. The E. coli RL211 cells expressing each construct were spotted on LB-agar medium with (+) or without (-) chloramphenicol (Cm), and the cells were incubated at 37°C.