Synthetic versions of firefly luciferase and Renilla luciferase reporter genes that resist transgene silencing in sugarcane

Table 1 Fold expression changes in multiple tissues assayed from pUbi- luc and pUbi- luc* transgenic lines of sugarcane

Luminometer assay	pUbi-*luc*Mean +/- SD	pUbi- *luc** Mean +/- SD	Mean fold difference
Plantlets	20040 +/- 8311	2215000 +/- 979000	110x
Leaf +3	264000 +/- 205500	4829000 +/- 1770000	18x
Leaf -1	52730 +/- 23730	7030000 +/- 2913000	133x
Meristem-Internode 2	139.4 +/- 105.8	1031000 +/- 447200	7396x
Internode 6	1455000 +/- 869400	33430000 +/- 10180000	23x
Root	302800 +/- 82140	42080000 +/- 12360000	139x
Camera assay
Internode 3	6.619 +/- 3.353	5312 +/- 3249	802x
Internode 4	56.70 +/- 19.09	6430 +/- 2925	113x
Internode 5	144.3 +/- 64.86	6033 +/- 2029	42x
Internode 6	165.2 +/- 68.15	5680 +/- 1937	34x
Internode 7	231.6 +/- 85.04	4203 +/- 1077	18x
Internode 8	323.6 +/- 109.7	3470 +/- 813.3	11x
Internode 9	440.3 +/- 137.4	3045 +/- 663.4	7x
Internode 10	397.8 +/- 123.9	3014 +/- 698.4	8x
Internode 12	441.3 +/- 116.5	3296 +/- 737.6	7x
Internode 14	421.5 +/- 107.8	4047 +/- 1093	10x
Internode 16	414.4 +/- 90.91	3757 +/- 1057	9x
3^rd basal Internode	601.5 +/- 155.5	5472 +/- 1287	9x

Except for the tissue cultured plantlets, assays were performed on tissues from maturing plants at the ~20 internode stage of development.

ISSN: 1471-2229