Diagrammatic representation of artificial microRNA constructs. (A) Linear structure of the miR168a primary transcript gene (MIR168a, nt 120 to 355, GenBank Accession No. EU549054.1). Sequences of the miR168a and its complementary region (illustrated as approximately miR168a*) in the gene are displayed in the boxes. (B) Structure of the binary vectors for expression of amiRNAs. Construct names are indicated at the left. The sequences of the designed amiRNA and its complementary region (approximately amiRNA*) are displayed in the boxes. 35S-P, CaMV 35S promoter. 35S-T, CaMV 35S terminator.