Predicted amino acid sequences of the eight tomato Pht1 genes LePT1 to 8 . Sequence alignment analysis was carried out using multiple alignment algorithm wrapped within the DNAMAN 7.0 program (http://www.lynnon.com/). Identical amino acids are shaded and gaps are indicated by dots. The consensus sites for phosphorylation by protein kinase C and casein kinase II are shown by two arrowheads with red colour and the conserved N-glycosylation residue is shown by a green arrowhead. The characteristic Pht1 signature was underlined. The transmembrane domains (broken underline) were predicted by the Toppred algorithm (http://bioweb.pasteur.fr/seqanal/interfaces/toppred.html).