Figure 2

SLK1 and SLK2 interact with LUFS domain of LUH in Yeast Two Hybrid and in planta . (A) LUH was expressed from bait vector as BD fusion and SLK1 or SLK2 from prey vector as AD fusion. Results of yeast two hybrid assay indicating the activation of α- Galactosidase reporter expression. Blue color indicates positive interaction. (B) The LUFS domain of LUH (amino acid residues 1-88) was expressed as BD fusion and SLK1 or SLK2 as AD fusion that is sufficient for interaction with SLK1 and SLK2. (C) Arabidopsis protoplasts were transfected with 15 μg of each plasmid containing LUH, LUFS domain as N-LUC fusion and SLK1 or SLK2 as C-LUC fusion. CaMV 35S::Renilla LUC reporter was used as an internal control for transfection. LUC activity is expressed relative to the R-LUC activity. Error bars are SE (n = 3). Asterisks indicate values that are significantly different from the vector control (*P <0.05, Student’s t test).