Figure 2
Phylogenetic analysis of EsDREB2B and homologous proteins. Phylogenetic tree of subtype1 (DREB2-type) transcription factors in E. songoricum (EsDREB2B, pink and bold), soybean (red), Arabidopsis (blue), rice (cyan) and other deduced polypeptides with high sequence identities with EsDREB2B. The neighbor-joining tree is based on an alignment of the complete deduced polypeptide sequences (residues 1–210). Bootstrap values from 1000 replicates were used to assess the robustness of the tree. Solid box (■) indicated truncated sequences clade with CMIV-4 motif. Bootstrap values >50 are shown. EsDREB2B (ADV57357.1) is from E. songoricum; CAP2 (ABC49682.1) is from chickpea; AtDREB2A (AAL36328.1), AtDREB2B (AAP37710.1), AtDREB2C (NP_565929.1), AtDREB2E (NP_181368.1), AtDREB2H (NP_181566.2) are from Arabidopsis; GmDREB2A;1 (AFU35562.1), GmDREB2A;2 (AFU35563.1), GmDREB2B;1 (AAQ57226.1), GmDREB2B;2 (AAT12423.1), GmDREB2C;1 (NP_001236509.1), GmDREB2C;2 (AAP83131.1), GmDREB2D;1 (AFU35564.1), GmDREB2D;2 (AFU35565.1) are from soybean; OsDREB2A (NP_001042107.1) and OsDREB2B (NP_001055260.2) are from rice; in addition, other protein accession numbers are as follows: EsDREB2B (ADV57357.1); MtDREB2B (AFK38091.1); CAP2 (ABC49682.1); MtDREB2C (XP_003597059.1); LjDREB2C (AFK47592.1); SdDREB2C (AFP89590.1); PpDREB2C-like (EMJ17611.1); FvDREB2C-like (XP_004293490.1); StDREB (AEI98833.1); SlDREB (NP_001234689.1); RcDREB2C (XP_002520794.1); PeDREB2 (ABY19375.1); SbDREB2 (AFI71292.1); BdDREB2B-like (XP_003568655.1); AcDREB (ACX94337.1); TaDREB3B (AAX13278.1). Blue = A. thaliana, Red = G. max, Turqoise = O. sativum and Purple = E. songoricum.