JA responsiveness of LeMYC2 transgenic plants. A, The root phenotype of 14-day-old constant WL (90 μmol/m2/s) grown various transgenic lines of tomato with control (VEC) grown in presence of 10 μM JA. From left to right, plants of VEC (vector control), LeMYC2OE and LeMYC2Ri1 lines are shown. B and C, Quantification of number of lateral roots (B) and root length (C), respectively, of those shown in A. The experiments were repeated thrice and similar results were obtained, the data shown is the representative of one of those experiments. The error bars indicate SD (n = 15). D and E, Real-time PCR analysis of JA marker genes, LIN6 (D) and PIN2 (E) from 6-day-old controls (UN and VEC) and various LeMYC2 transgenic lines in WL (60 μmol/m2/s) and transferred to MS or MS with 50 μM JA for 6 h in WL. The error bars indicate SD (n = 3), normalized with LeACTIN. Accession numbers of genes are LIN6 (AF566005) and PIN2 (BG629234). F, The model shows the function of MYC2 in tomato (LeMYC2) and Arabidopsis (AtMYC2).