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Figure 1 | BMC Plant Biology

Figure 1

From: Genome-wide data (ChIP-seq) enabled identification of cell wall-related and aquaporin genes as targets of tomato ASR1, a drought stress-responsive transcription factor

Figure 1

Library construction and quality assessment of the antibody. A) Determination of the DNA fragment sizes in input chromatin samples by agarose gel electrophoresis. The gel was stained with ethidium bromide and the band intensity was quantified with ImageJ software. M indicates molecular weight markers (100-bp ladder). B) Western blot of the ASR1-immunoprecipitated DNA samples subjected to the ChIP protocol (without crosslinking) using the pure specific anti-ASR1 antibody (ASR1) or an irrelevant, non-specific antibody (NS) and a secondary antibody that recognizes only native immunoglobulins to prevent the undesired detection of immunoglobulins previously used for immunoprecipitation (which denatured on the gel during electrophoresis). INP indicates the input chromatin sample.

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BMC Plant Biology

ISSN: 1471-2229

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