Figure 2
Quantification of cytokinin response strength in ARR1 overexpressing lines. (A) Representative ten-day-old arr1-1, 35S:ARR1 (WT-ox) and 35S:ARR1D94E(PM-ox) seedlings grown on MS/2 medium. The arrows point at the root tips. (B) Quantification of root elongation growth. Three-day-old transgenic seedlings grown on MS/2 plates were transferred to fresh MS/2 plates. In parallel, three-day-old Col-0 and arr1-1 seedlings were transferred MS/2 plates containing the indicated concentrations of BA. The root length of each seedling was marked at the moment of transfer (initial length) and after 7 days of growth on test media (final length). Root elongation (difference between final and initial root length) of 30 seedlings per line is presented as a dose–response curve for Col-0 and arr1-1 and as bars (mean ± SD) for the transgenic lines. The results were analyzed using one-way analysis of variance (ANOVA) followed by Tukey's multiple comparisons test. The Col-0 and arr1-1 root lengths that did not significantly (ns) differ from the root length of the transgenic line are encircled.