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Table 2 Amino acid content at different HR phases

From: Context of action of Proline Dehydrogenase (ProDH) in the Hypersensitive Response of Arabidopsis

 

wild type

p5cdh

 

C

I

II

III

C

I

II

III

Ser

872 ± 129

840 ± 160

948 ± 98

1387 ± 150

895 ± 96

1013 ± 65

891 ± 19

820 ± 70

Arg

7.2 ± 0.9

11.2 ± 0.5*

19 ± 4*

62 ± 7*

7.1 ± 0.9

9.1 ± 1.2*

19 ± 4*

34 ± 6*

Tyr

10 ± 1

21 ± 1*

34 ± 6*

78 ± 7*

11 ± 1

17 ± 1*

39 ± 3*

33 ± 5*

Val

59 ± 8

173 ± 17*

171 ± 14*

440 ± 76*

59 ± 4

102 ± 10*

188 ± 22*

223 ± 44*

Phe

28 ± 5

98 ± 6*

108 ± 22*

272 ± 36*

29 ± 3

56 ± 4*

118 ± 12*

127 ± 27*

His

13 ± 2

26 ± 5*

37 ± 6*

87 ± 16*

16 ± 3

28.7 ± 1.4*

35 ± 5*

38 ± 4*

Ile

17 ± 4

56 ± 10*

58 ± 12*

228 ± 45*

20 ± 2

40 ± 3*

78 ± 7*

50 ± 7*

Lys

9 ± 2

20 ± 4*

23 ± 5*

92 ± 19*

10 ± 2

18 ± 4*

38 ± 5*

#

Leu

25 ± 3

95 ± 8*

115 ± 24*

408 ± 59*

22 ± 3

51 ± 9*

128 ± 9*

169 ± 36*

Gly

230 ± 38

280 ± 48*

368 ± 42*

708 ± 31*

284 ± 17

259 ± 7*

588 ± 32*

531 ± 74*

Asn

75 ± 16

124 ± 5*

145 ± 20*

134 ± 24*

90 ± 15

95 ± 18

128 ± 19

158 ± 17*

Thr

300 ± 43

497 ± 35*

449 ± 46*

733 ± 74*

366 ± 55

459 ± 32*

464 ± 60*

#

Ala

337 ± 46

433 ± 87*

391 ± 45*

618 ± 28*

318 ± 23

410 ± 31*

425 ± 15*

299 ± 57

GABA

19 ± 2

30 ± 3*

164 ± 23*

11 ± 1*

13 ± 3

19 ± 4*

291 ± 37*

4 ± 1*

Glu

693 ± 60

701 ± 49

812 ± 89

279 ± 49*

742 ± 83

833 ± 77

684 ± 51

523 ± 43*

Asp

285 ± 41

228 ± 48

300 ± 24

62 ± 15*

324 ± 8

401 ± 12*

202 ± 26*

114 ± 12*

P5C

1.3 ± 0.2

1.4 ± 0.3

1.4 ± 0.3

1.6 ± 0.2

1.2 ± 0.2

1.3 ± 0.1

1.9 ± 0.3*

1.4 ± 0.3

Pro

606 ± 106

376 ± 74*

572 ± 109

1393 ± 169*

995 ± 134 a

951 ± 201

941 ± 87

1112 ± 22*

Gln

916 ± 100

1114 ± 239*

718 ± 31*

764 ± 92

785 ± 70

838 ± 89*

836 ± 36

437 ± 97*

  1. C:uninfected samples (control); I, II, III: Pst-AvrRpm1-infected samples isolated at phases I, II or III of HR.
  2. *Significant differences between infected and control samples of the same genotype (p < 0.05 by t test).
  3. #Samples analyzed in different experiments containing similar values similar to those of wild type plants.
  4. aSignificant differences regarding the wild type control value (p < 0.05 by t test).
  5. Values are expressed as nmol/g fresh weight. Each value is mean ± SD of 3 independent experiments (10 leaves per plant, 6 plants per experiment).
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BMC Plant Biology

ISSN: 1471-2229

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