Validation of filtering method in identifying high confident regulatory interactions. (A) The percentages of experiment-confirmed regulatory interactions in regulatory interactions predicted by different data. To validate the filtering strategies of AthaMap data, the proportion of direct regulatory interactions (AGRIS) in the predicted ones (AthaMap) across different data filtering strategies were computed with the regulatory interactions of five TFs: FUS3 (AT3G26790), AtLEC2 (AT1G28300), AG (AT4G18960), AGL15 (AT5G13790) and HY5 (AT5G11260) present in both AGRIS and AthaMap. (B) Literature evidences about the function of TFs’ target pathways that were consistent with phenotypes of TFs’ mutant. The function of TFs’ target pathways were mined in literatures. The phenotypes of TFs’ mutant were obtained from AtPID (Arabidopsis thaliana Protein Interactome Database). The percentages of target pathways whose functions were consistent to TFs’ mutant phenotypes were computed.