PenB_MT2 gene, its mRNA isoform structures, RT-qPCR analysis of mRNA isoforms abundance, and a model of the Pen_MT2 protein secondary structure. (A) Schematic representation of the PenB_MT2 gene and its five mRNA isoforms. All designations are the same as in Figure 4. Real-time PCR analysis for the quantification of five alternatively spliced isoforms of PenB_MT2 gene in P. endiviifolia sp B female thalli-producing archegonia (B). Material was collected in the third season (2008) from the natural habitat. Calculation shows mean ± SD from three technical replicates. (C) Amino acid sequence of predicted PenB_MT2 protein. Transmembrane (tmm) and secondary structure (ss) prediction represent consensus prediction from GeneSilico metaserver; black barrels represent α-helises and white arrows represent β-sheets. Intrinsically unstructured residues (disorder) were predicted by GeneSilico MetaDisorder.