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Table 3 Functional classification of down-regulated proteins

From: Proteomic analysis of proteins related to rice grain chalkiness using iTRAQ and a novel comparison system based on a notched-belly mutant with white-belly

Accession

Protein Name

Score

Mass (kDa)

Cov. (%)

Unique peptide

C2/C1

T2/T1

C/T

Amino acid metabolism

       

gi|584706

Aspartate aminotransferase, cytoplasmic

320

49.8

15.7

6

0.788

1.150

0.685

gi|113535557

Putative glycine decarboxylase complex H-protein

117

20.8

15.2

2

1.055

1.308

0.807

Carbohydrate metabolism

       

gi|222640045

Putative aconitate hydratase, cytoplasmic

926

120.0

9.6

6

0.208

0.821

0.253

gi|28190676

Transketolase, chloroplastic

221

92.7

9.2

4

0.065

0.137

0.474

gi|113534863

Putative xylanase inhibitor

123

49.4

8.8

3

0.637

0.872

0.731

gi|108885236

Glucose and ribitol dehydrogenase homolog

276

37.3

23.7

6

1.015

1.288

0.788

gi|75114635

Probable alpha-glucosidase

676

102.6

5.1

4

1.135

1.364

0.832

gi|108707955

2,3-bisphosphoglycerate-independent phosphoglycerate mutase (PGAM)

261

72.8

6.1

1

0.040

-

-

Cell defense

        

gi|75298023

17.9 kDa class I heat shock protein

301

22.5

17.4

2

1.147

1.756

0.653

gi|1169521

Embryonic abundant protein 1

151

12.3

35.8

3

1.792

2.271

0.789

gi|113648660

Os12g0147200

327

19.1

32

3

1.048

1.299

0.807

Energy production

       

gi|113537902

Cytochrome b5 domain-containing protein-like

279

13.3

55.9

4

1.207

1.786

0.676

Lipid metabolism

       

gi|113623031

Acyl-CoA-binding protein

200

13.5

54.9

2

1.660

2.026

0.819

Protein synthesis, folding and degradation

       

gi|113549785

60S ribosomal protein L13a-4

85

31.1

6.3

1

0.541

1.081

0.500

gi|113624448

Cysteine proteinase 3 (Fragment)

182

48.9

13

3

1.177

1.663

0.708

gi|548770

60S ribosomal protein L3

64

61.3

6.7

2

0.169

0.238

0.710

gi|113649731

Ubiquitin-conjugating enzyme E2 variant 1C

238

19.5

29.5

2

0.734

1.031

0.712

gi|113535206

Dnak-type molecular chaperone Bip

4150

92.7

42.7

21

0.696

0.961

0.724

gi|556560

26S protease regulatory subunit 6A homolog

413

58.0

16.6

4

0.500

0.690

0.725

gi|113535132

RuBisCO large subunit-binding protein subunit beta, chloroplastic (Fragment)

791

80.6

20.1

4

0.566

0.777

0.728

gi|222642001

Putative UIP2 (SKP1-like 2, UFO-binding protein 2)

153

23.7

27.4

2

0.423

0.545

0.776

gi|75322635

Protein disulfide isomerase-like 2-3

289

60.2

15.6

5

0.817

1.018

0.803

gi|113579164

Luminal-binding protein 2/B-70/Bip 2

454

86.3

4.1

3

0.579

0.718

0.806

Redox homeostasis

       

gi|113579377

Aldose reductase

222

44.4

27.7

6

1.183

1.575

0.751

gi|113550330

Putative oxidoreductase

97

42.0

9.9

2

0.789

1.030

0.766

gi|52076516

Thioredoxin O, mitochondrial

65

24.5

14.3

2

0.508

0.629

0.808

gi|516839

Catalase isozyme B

319

64.3

22.2

5

0.719

0.878

0.819

gi|119370643

Glutaredoxin-C8

398

18.8

44.9

6

0.680

0.824

0.825

gi|29367419

Peptide methionine sulfoxide reductase B5

265

21.3

29.8

3

1.188

1.437

0.827

Unknown pathway

       

gi|284431762

Prolamin PROL 17D

356

18.6

35.9

1

0.435

1.319

0.330

gi|62733566

Os03g0723400

335

14.3

70.9

3

0.581

1.683

0.345

gi|306415963

Glycine-rich RNA-binding protein 1 (Fragment)

2043

17.0

41

1

0.496

1.351

0.367

gi|122222504

Protein SGT1 homolog

302

52.6

7.1

2

0.512

1.148

0.446

gi|113624371

Os08g0543600

98

104.8

3.7

2

0.625

1.182

0.529

gi|113536747

Os02g0576400

123

14.0

26.9

2

0.886

1.655

0.535

gi|113537457

Os02g0715400

298

11.9

32.7

2

0.848

1.440

0.589

gi|28564794

Putative uncharacterized protein P0534A03.117

258

25.8

24.4

3

0.792

1.342

0.590

gi|255672911

Vegetative cell wall protein gp1-like

108

66.4

8.1

3

0.979

1.430

0.685

gi|113578822

Ran-binding protein 3

82

63.4

4.9

2

1.220

1.719

0.710

gi|77556700

Os12g0626500

1606

16.3

38.6

3

1.019

1.396

0.730

gi|113537680

Annexin D7

144

42.1

13.4

4

0.747

0.951

0.785

gi|113533811

Putative calcium-binding protein

245

72.6

8.6

4

1.015

1.290

0.787

gi|18652814

Zinc finger protein ZAT7

102

19.7

8.8

1

1.165

1.420

0.820

  1. Note: “-”, not detected.