Figure 6

cycam1-1 and cycam1-2 accumulate more reactive oxygen species (ROS) than the WT after A. brassicae infection (A) or application of the A. brassicae Tox (B). Roots of twelve-day old WT and cycam1 seedlings were inoculated with 5 μl A. brassicae spore suspension containing 10⁴ to 10⁵ cfu ml⁻¹(A) or with the A. brassicae Tox (B) and the ROS levels were measured in the roots 2 days later. Relative expression of ROS marker genes (C) or ROS scavenging genes (D) in WT and cycam1 roots 2 days after infection with A. brassicae spores as described for (A). The annotated functions of the genes/proteins are given in brackets [H₂O₂; singlet oxygen, (¹O₂); superoxide anion radical, (${\mathrm{O}}_2^{.-}$)]. For gene abbreviations, cf. TAIR (http://www.arabidopsis.org/). The mRNA levels of mock-treated WT seedlings were taken as 1.0 and the other values are expressed relative to it. All values are means ± SEs relative to the level of the root GAPDHC mRNA levels. Based on 3 independent experiments with 24 seedlings per experiment. Asterisks indicate significant differences, as determined by Student’s t-test (* P < 0.05; ** P < 0.01).