Figure 4

Phenotypes and molecular analysis of OsMYB103L overexpressing plants (OE). (A) OsMYB103L expression in OE as determined by qRT-PCR. Rice ACTIN1 gene was used as internal control. Each column represents an average of three replicates and bars indicate the SDs. (B) Morphology of 2-month-old WT and OE leaves. OE showed inward rolled leaves compared to WT (top panel); Cross sections of leaves of WT and OE (bottom panel). Bars = 10 cm (top panel) or 3 mm (bottom panel). (C) Statistical analysis of LRIs at the time of flowering for three OE lines of the top three leaf blades (n = 20). Asterisks (***) indicate a significant difference between transgenic plants and WT controls at P < 0.001, by Student’s t-test. (D) Transverse sections of the flag leaves in the middle part of WT and OE. Shown are regions between two large veins. lv, large vein; sv, small vein. Bars = 50 μm.