Figure 6

Overexpression of GmAKT2 enhanced SMV resistance. (A) SMV symptoms in the WT and GmAKT2 overexpression plants at 35 DAI. Ten-day-old soybean plants in low-K soil with unrolled unifoliate leaves were mechanically inoculated with SMV or buffer (Mock). (B) SMV symptoms on the newest trifoliate leaves of Williams 82 and GmAKT2-overexpressing transgenic lines Oe1 and Oe2 at 14, 28, and 35 DAI. (C) Amount of SMV RNA detected by qRT-PCR. The middle leaflets of the leaves of Williams 82, and GmAKT2-ovexpression lines Oe1 and Oe2 were sampled at 14 and 28 DAI to extract total RNA for quantitative RT-PCR analysis. Transcript levels were calculated using the formula 2^-ΔCt for the expression levels relative to GmACTIN. Data represent means of three biological replicates with error bars indicating SD.