Figure 1

AtHB12 and AtHB7 expression levels fluctuate during development and in response to abiotic stress. (A) Total RNA was isolated from 3- and 14-day-old WT plants and from leaves of 21, 28, 38 and 45-day-old plants and analysed by qRT-PCR for AtHB7 and AtHB12 transcript levels with specific oligonucleotides (Additional file 4). (B) Total RNA was isolated from 14-day-old plants treated with 300 mM mannitol and analysed as in A. (C) RNA was isolated from leaves of 38-day-old plants subjected to a moderate water stress (MWS) starting at day 21 after germination, during 17 days. Transcript levels values were normalised with AtHB7 transcripts at day 3 in A or at time 0 in B and C, applying the ∆∆Ct method. Error bars represent SE calculated from three independent biological replicates. Actin transcripts (ACTIN2 and ACTIN8) were used as a reference. “*” , “a”, “b”, “c” and “d” denote statistical differences obtained with-ANOVA-Tukey’s P < 0.05.