NaTrxh: GFP is secreted in onion epidermal cells. (A – B) GFP fluorescence from the NaTrxh: GFP fusion protein, was localized on the cell wall (CW). (C – D) p11 is a known secreted protein in N. alata styles that was also secreted. (E – F) GFP alone was not secreted when transiently expressed. (G – H) Non-transformed cells. (A, C, E, G) GFP fluorescence. (B, D, F, H) Bright fields merged with flourescence images. M: plasma membrane; CW: cell wall; GFP fluorescent signal (arrows). The cells were plasmolized with 1.0 M NaCl before confocal observation. Scale bars = 30 μm.