NaTrxh localized to the cell wall or associates to secretory elements in N. alata styles. (A) Semi-quantitative analysis of the localization of the gold particles (i.e., NaTrxh) by the electron microscopic immune-gold assays. Sub-panel shows NaTrxh was immunodetected in a stylar microsomal fraction (Mi) along with vATPase. E: crude protein extract. (B) NaTrxh was associated with vesicles (V), the trans-Golgi network (TGN), or in the cell wall (CW). (C – D) NaTrxh was mainly found associated to membranous systems, such as the endoplasmic reticulum (ER), the Golgi apparatus (G), or within vesicles. (E – F) Vesicles containing gold particles. In (f), a vesicle is observed fused to the plasma membrane (M). NaTrxh localization (arrows). Scale bars are shown in each micrograph. (B – F) Ultra thin sections of N. alata styles were treated with anti-NaTrxh and then with anti-rabbit coupled to gold particles.