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Figure 4 | BMC Plant Biology

Figure 4

From: Differential activation of sporamin expression in response to abiotic mechanical wounding and biotic herbivore attack in the sweet potato

Figure 4

Isolation of Ib NAC 1 and Ib WRKY 1 proteins interacting with SWRE DNA by yeast one-hybrid screening. Two cDNA clones (IbNAC1 and IbWRKY1) were isolated by Y1H screening, and re-transformed into the yeast strain Y187 in the presence of plasmid containing three tandem repeats of the SWRE sequence (3xSWRE-pHIS2) (A) or without 3xSWRE3 (pHIS2). Growth of yeast transformants is shown on both + His (A) and -His/100 mM 3-AT medium (B). Positive control and negative control are employed to standardize selection condition by using yeast strain Y187 co-transformed with pGAD-Rec2-53/p53HIS2 or pGAD-Rec2-53/pHIS2 (Clontech).

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