Figure 2

The effect of changes in temperature, up to eight days before inoculation with Pst, on yellow rust resistance in adult plants of the wheat line UC1041 - Yr36. A) Mean percentage (%) leaf area covered in uredia 18 dpi on adult plants inoculated with Pst isolate 08/21 kept at 12°C/18°C or 12°C/25°C pre and post inoculation (dark grey) or transferred to the other temperature regime post-inoculation (light grey). Mean values (± 1 standard error) were obtained from three independent experiments. Different letters indicate statistical significant differences (P < 0.01). B) Pst symptoms on flag leaves at 18 dpi in the four temperature regimes. Uredia can be observed within necrotic/chlorotic areas. C-D) Mean percentage (%) leaf area covered in uredia at 18 dpi on adult plants inoculated with Pst isolate 08/21 and kept at the original temperature regime of C) 12°C/18°C or D) 12°C/25°C pre and post inoculation (dark grey) or shifted at various time points before inoculation to the other temperature regime (light grey). Mean values (± 1 standard error) were obtained from four biological replicates. Different letters indicate statistically significant differences (P < 0.01).