| Host plant |
---|
Sequencing |
Z. mays
|
M. truncatula
|
---|
Total raw sequence | 2.73 Gbp | 2.91 Gbp |
Reads Total
|
35,894,662
|
38,228,134
|
Host reads | (401,352) | (1,588,592) |
Host filtered | 35,493,310 | 36,639,542 |
Quality trimmed | 26,947,737 | 27,325,845 |
Assembly
| | |
Assembly length | 12.77 Mbp | 12.25 Mbp |
Unigenes Total
|
28,126
|
26,709
|
Unigenes >500 bp | 9,369 | 9,718 |
Min/Max length (bp) | 197/3,115 | 197/3,265 |
N50 | 525 bp | 536 bp |
N50 >500 bp | 731 bp | 695 bp |
Annotation
| | |
Host unigenes | (4,967) | (7,785) |
Non-plant unigenes | (127) | (329) |
Triphysaria unigenes
|
23,032
|
18,595
|
Triphysaria hits | 17,887 | 14,352 |
Other Plant hits | 2,975 | 2,086 |
No hits | 2,170 | 2,157 |
- Low quality reads were filtered before assembly, and host sequences were filtered both before and after assembly. Unigenes remaining after removal of host plant and non-plant sequences were aligned with BLASTx to sequences detected in any other PPGP transcriptome library of Triphysaria versicolor (http://ppgp.huck.psu.edu/). Unigenes with less than 95% pairwise identity to either host or to other Triphysaria libraries were sorted further if a BLASTx search of the NR (http://www.ncbi.nlm.nih.gov) database yielded alignments of 1e-10 or stronger. The remaining unclassified unigenes were submitted to OrthoMCL DB and InterProScan. Unigenes that remained unclassified after the final screen are called “no hit” unigenes.