SBGN Pathway of heterotrophic metabolism and changes in expression of metabolic genes in Sitka spruce needles during winter hardening. Metabolic pathways were drawn using the BEACON tool to represent activities as boxed reactions with associated temporal changes in expression of relevant genes (rows in colored bins/small boxes) in TP1 through TP4 obtained from MapMan. Gray boxes indicate that the change in gene expression was not statistically significant, while blue and red indicate a statistically significant up- and down-regulation, respectively (P ≤ 0.05). Arrows connecting the activities indicate positive influence to generate a metabolic network, while “T” represents an inhibitory effect, e.g., components of the alternative ETC. in mitochondria interfere with adenosine triphosphate (ATP) synthesis. Each activity is associated with an enzyme (white rounded rectangle), enzyme complex (light gray octagon), or transporter (dark gray rounded rectangle). Abbreviations: 3PGK, 3-phosphoglycerate kinase; AGPase, ADP-glucose pyrophosphorylase; AOX, alternative oxidase; cyt, cytochrome; DH, dehydrogenase; FBPase, fructose-1,6-bisphosphatase; Fru, fructose; G3PDH, glyceraldehydes-3-phosphate DH; ICDH, isocitrate DH; KGDH, α-ketoglutarate DH; MDH, malate DH; ME, malic enzyme; NAD(P), oxidized nicotinamide adenine dinucleotide (phosphate); NAD(P)H, reduced nicotinamide adenine dinucleotide (phosphate); NP, non-phosphorylating; P, phosphate; PEP, phosphoenolpyruvate; PEPC, PEP carboxylase; PDH, pyruvate DH; PFK, phosphofructokinase; PK, pyruvate kinase; Pi, inorganic phosphate; PPi, pyrophosphate; PPDK, pyruvate phosphate dikinase; SDH, succinate DH; SUS, sucrose synthase; TCA, tricarboxylic acid cycle; UCP, uncoupling protein; UGPase, UDP-glucose pyrophosphorylase; UQ/UQH2, oxidized/reduced ubiquinone.