Figure 6
From: Genome-wide identification and functional analyses of calmodulin genes in Solanaceousspecies
Silencing of SlCaM2 and SlCaM6 in tomato plants reduced resistance to Tobacco rattle virus (TRV). (A) Phenotypes of the SlCaM gene-silenced plants. Gene silencing analyses were performed using TRV-based vectors. TRV viral symptoms were more severe in SlCaM gene-silenced plants compared with the eGFP-control plants. Photographs were taken 3 weeks post agro-infiltration. (B) Detection of transcripts of the TRV1 replicase and TRV2 2b genes in silencing plants by qRT-PCR. (C) Evaluation of gene silencing efficiency. Expression levels of the SlCaM2 and SlCaM6 genes in tomato plants were examined by qRT-PCR. Significant difference between expression values within the target genes is indicated as different lowercase letters (P <0.05, DMRT), while no significant difference as “ns”.