Table 2 Specific primers used for the amplification of TaSK sequences in Nullisomic-Tetrasomic lines
From: Identification and characterization of two wheat Glycogen Synthase Kinase 3/ SHAGGY-like kinases
Primers | Sequences (5′-3′) | Target sequences |
|---|---|---|
SF97 | AGGCACATGATCAGTTCAATAAT | TaSK1-A |
SR96 | TATGTCTCCACCTCCTACATC | TaSK1-A |
SF61 | CCCAACGCAAGAGCAAAG | TaSK1-B |
SR98 | CCAGACGCGACATGAAATC | TaSK1-B |
SF99 | CTGCTAATGTATGTATCATCTGCT | TaSK1-C |
SR101 | AGGACATACTCGCAAGACTC | TaSK1-C |
SF104 | GATGTTACTTACCTATCATTTTTCTTGT | TaSK2-A |
SR105 | ACCTTGTGCCAAGGATGAG | TaSK2-A |
SF102 | CTCTTTAGCTATGACAACTCATTGA | TaSK2-B |
SR102 | TGAAAGACGATGCCAAACG | TaSK2-B |
dCAPS-T2C-F | GAGCTGCAGCTTATGCGTTCG | TaSK2-C |
dCAPS-T2C-R | AGATAAGTGGCATCCCCTGTTTGG | TaSK2-C |