Figure 3

Confirmation of genes or proteins altered in W64A o2 by qRT-PCR. Expression of the indicated genes was analyzed in 22 DAP endosperms of W64A + and W64Ao2 by GeneCalling transcript profiling (A) or qRT-PCR to confirm the difference in expression in the transcript profiling, or to measure the expression levels of genes that were differentially expressed by 2D SDS-PAGE analysis (B). All expression values are normalized relative to the expression of RRB1. Asterisks indicate significantly different expression using the two-tailed t-test at a level of p < 0.05. Note that the Y-axis is logarithmic to accommodate the wide differences in gene expression levels among the transcripts. Missing columns in A indicate that the gene was not among the genes that had a confirmed identity in the transcript profiling data.