Figure 2
PDQuest-generated master gel image showing the general pattern of matched protein spots from the total leaf proteome of grapefruit plants that were Las-infected or uninfected and pre-symptomatic or symptomatic for HLB. Labeled protein spots were differentially produced in response to Las-infection and described in Tables 1, 2, and 3. Two-year old healthy plants were either graft-inoculated with side shoots from PCR-confirmed Las-infected bud sticks or uninoculated and leaf samples were analyzed at three months post-inoculation (for pre-symptomatic plants) or six months post-inoculation (for symptomatic plants). A total of 200 μg of protein was loaded on a pH 4–7 IpG strip and protein spots were visualized by staining with Coomassie Brilliant Blue (CBB). Mr, relative molecular weight; pI, isoelectric point.