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Figure 4 | BMC Plant Biology

Figure 4

From: Homo-dimerization and ligand binding by the leucine-rich repeat domain at RHG1/RFS2 underlying resistance to two soybean pathogens

Figure 4

Far-Western analysis of soybean root proteins at 24 dap (10 dai) probed with the LRR domain of GmRLK18-1. Panel (A): Shown is a portion of a 2D gel (14.4-21.5 KDa; 7.5-10.0 pI) from 34–23 (resistant) SCN inoculated total root proteins with spots visualized with silver staining. Panel (B): Proteins transferred to a membrane and probed with purified GmRLK18-1 LRR domain and 6X his-RHG1. Anti-His-HRP was used as the secondary probe. The single spot identified (arrowed) was excised from the duplicate gel and analyzed by Q-TOF (MS-MS) to identify a cyclophilin as a GmRLK18-1 LRR domain interacting partner.

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BMC Plant Biology

ISSN: 1471-2229

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