Figure 6

A. DNA sequence alignments of some Twinkle primase domain conserved regions to show the extent of changes between different organisms. The DNA sequences for the Twinkle protein from T7, Arabidopsis (At1g30680), human (Hs) and Drosophila (Dm) are shown for the conserved motifs I, IV and V. The locations of the cysteine residues in Motif I are indicated above the sequence while the corresponding codon sequence is underlined in the DNA sequence. The central conserved elements of each motif are shaded yellow. Base differences from the T7 gp4 sequence are shaded dark blue with white lettering.