Table 1 Primers used for the expression analysis of phenylpropanoid pathway genes by using RT q-PCR [[14]]
Gene abbreviation | Gene definition | Gen Bank accession | Unigene ID | Primer pair |
|---|---|---|---|---|
5′-Forward-3′/5′-Reverse-3′ | ||||
STS1 | Stilbene synthase 1 | DO366301 | Vvi.8 | CGAAGCAACTAGGCATGTGT/ |
CTCCCCAATCCAATCCTTCA | ||||
PAL1 | Phenylalanine ammonialyase | EC987386 | Vvi.1950 | CCGAACCGAATCAAGGACTG/ |
GTTCCAGCCACTGAGACAAT | ||||
C4H1 | Cinnamate-4-hydroxylase | EC995763 | Vvi.6228 | AAAGGGTGGGCAGTTCAGTT/ |
GGGGGGTGAAAGGAAGATAT | ||||
4CL1 | 4-coumarate-CoA ligase | EC947790 | Vvi.1251 | CTGATGCCGCTGTTGTTTCG/ |
GCAGGATTTTACCCGATGGA | ||||
CHS1 | Chalcone synthase1 | EC996578 | Vvi.117 | GTCCCAGGGTTGATTTCCAA/ |
TCTCTTCCTTCAGACCCAGTT | ||||
CCR2 | Cinnamoyl-CoA reductase | CF517687 | Vvi.15864 | ACAGCATGACGACTCTCTTCG/ |
AGTGACAAGGGGTGGATTGA |