Genome size expansion and the relationship between nuclear DNA content and spore size in the Asplenium monanthes fern complex (Aspleniaceae)

Dyer, Robert J; Pellicer, Jaume; Savolainen, Vincent; Leitch, Ilia J; Schneider, Harald

doi:10.1186/1471-2229-13-219

Table 2 A summary of the specimens analysed including, mean 2C DNA content and mean spore length measurements per specimen (both with standard deviation values)

From: Genome size expansion and the relationship between nuclear DNA content and spore size in the Asplenium monanthes fern complex (Aspleniaceae)

Species	Voucher	Mean spore length (μm)	Mean 2C-value (pg)	CV%	Reproductive mode
A.fibrillosum*	RD10b	47.90 ± 8.14	37.78 ± 0.18	6.95	Sexual
A.formosum	RD28	27.85 ± 1.26	13.46 ± 0.03	3.77	Sexual
A.formosum	RD33	32.20 ± 3.80	13.19 ± 0.03	2.73	Sexual
A.formosum†	RD157 (IJ2436)	34.65 ± 3.37	13.93 ± 0.23	3.15	Sexual
A.formosum†	RD158 (ES1398)	33.56 ± 3.78	13.87 ± 0.11	2.24	Sexual
A.hallbergii	RD23	48.69 ± 5.18	26.05 ± 0.06	3.72	Apomictic
A.aff.hallbergii	RD90	43.32 ± 3.10	26.17 ± 0.12	3.47	Apomictic
A.hallbergii	RD93 (RD112)	47.63 ± 4.81	26.23 ± 0.19	3.40	Apomictic
A.hallbergii	RD111	41.33 ± 3.91	26.00 ± 0.06	3.24	Apomictic
A.aff.heterochroum	RD9a	38.62 ± 3.35	18.67 ± 0.08	3.11	Apomictic
A.aff.heterochroum	RD75	37.43 ± 3.19	14.38 ± 0.05	2.39	Apomictic
A.monanthes (MO1) I	RD70	39.03 ± 3.35	30.72 ± 0.78	3.94	Apomictic
A.monanthes (MO1) I	RD101b	39.44 ± 2.96	29.68 ± 0.12	4.19	Apomictic
A.monanthes (MO1) I	RD104	41.85 ± 3.73	30.11 ± 0.32	4.15	Apomictic
A.monanthes (MO1) II	RD80	43.15 ± 4.53	28.03 ± 0.06	2.75	Apomictic
A.monanthes (MO1) II	RD94	45.79 ± 3.31	27.96 ± 0.19	3.36	Apomictic
A.monanthes (MO1) III	RD1a	48.54 ± 5.94	26.54 ± 0.16	4.05	Apomictic
A.monanthes (MO1) III	RD17	40.31 ± 3.34	26.84 ± 0.52	4.35	Apomictic
A.monanthes (MO1) III	RD20	45.53 ± 3.38	26.45 ± 0.39	3.77	Apomictic
A.monanthes (MO1) III	RD73	38.09 ± 2.42	26.91 ± 0.14	4.08	Apomictic
A.monanthes (MO1) III	RD76	40.78 ± 5.07	27.21 ± 0.13	3.82	Apomictic
A.monanthes (MO1) III	RD89	45.30 ± 3.24	26.82 ± 0.02	3.14	Apomictic
A.monanthes (MO1) III	RD103	46.39 ± 3.54	26.90 ± 0.14	3.64	Apomictic
A.monanthes (MO1) III	RD110	44.54 ± 2.85	26.62 ± 0.16	4.04	Apomictic
A.monanthes (MO1) III	RD132	43.97 ± 4.27	27.41 ± 0.26	4.03	Apomictic
A.monanthes (MO2) I	RD53 (RD45)	47.76 ± 4.27	27.40 ± 0.09	3.53	Apomictic
A.monanthes (MO2) I	RD99	48.54 ± 5.88	26.09 ± 0.24	5.29	Apomictic
A.monanthes (MO2) I	RD125	42.80 ± 4.36	27.43 ± 0.18	4.39	Apomictic
A.monanthes (MO2) I	RD135	38.94 ± 3.69	27.32 ± 0.06	3.92	Apomictic
A.monanthes (MO2) II	RD96	42.11 ± 4.82	18.58 ± 0.09	3.00	Apomictic
A.polyphyllum*	RD98 (RD95)	35.42 ± 3.58	29.95 ± 0.10	4.02	Sexual
A.resiliens	RD128	45.07 ± 2.96	13.90 ± 0.07	2.75	Apomictic
A.resiliens	RD63	42.39 ± 2.93	13.70 ± 0.12	2.80	Apomictic
A.resiliens	RD107	43.45 ± 4.35	14.50 ± 0.04	3.07	Apomictic
A.resiliens	RD127 (RD64)	42.26 ± 4.59	13.87 ± 0.07	2.85	Apomictic
A.soleirolioides*	RD82 (RD71)	39.37 ± 2.89	50.29 ± 0.16	4.03	Sexual
Spec.nov.1†	RD162 (JM1339)	30.87 ± 2.80	17.67 ± 0.19	4.26	Sexual
Spec.nov.2†	RD163 (SK10151)	29.71 ± 2.63	22.20 ± 0.28	3.54	Sexual

Voucher numbers are linked to accession numbers for sequences deposited at Genbank [29]. In cases where alternate voucher numbers appear in brackets, these accessions were used for phylogenetic analysis. CV% indicates the mean coefficient of variation of the 2C DNA peak for each specimen. Reproductive mode was determined from spore number per sporangia, and some prothalli observations [29]. MO1 and MO2 refer to the two clades within the A.monanthes complex identified by the phylogenetic analysis of Dyer et al. [29].
*Data for these species (which belong to the A. castaneum clade) were not considered to be reliable as they were estimated from 2 year old silica dried material and were therefore not included in analysis.
†Data for these species were estimated from 6 month old silica dried material.

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ISSN: 1471-2229

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