Figure 1

RS activity in crude extracts from E. coli transformed with RS5 :pPROExHTc (lower chromatogram). Crude extracts were incubated with 100 mM Suc and 10 mM Gol at pH 7.5 for 1 h. The empty vector control, pPROEx HTc, did not show any RS activity (upper chromatogram). Raf, raffinose (7.2 min); Suc, sucrose (8.8 min); Gol, galactinol (11.2 min); Ino, myo-inositol (13.9 min). The Raf biosynthetic reaction yields Ino consequent to the transfer of a galactose moiety from Gol to Suc.