Expression of LgDes results in the accumulation of GLA and SDA in N. benthamiana leaves. Portions of gas chromatogram traces for FA-pyrrolidide derivatives from Nicotiana benthamiana containing empty vector control (Panel A), LgDes (Panel B), borage Δ6-desaturase (Panel C). Peak labeled 1, corresponds to LA, 2, ALA, 3, GLA, 4, SDA, and 5, 20:0. Mass spectra corresponding to the pyrrolidide derivatives of GLA (Panel D), and SDA (Panel E) derived from the expression of LgDes shown in Panel B, peaks 3 and 4, respectively, are shown. Ions diagnostic for the positions of the double bonds in the acyl chains and the mass ions are indicated and diagrammed according to W.W. Christie (http://lipidlibrary.aocs.org/ms/masspec.html).