The qPCR analysis of CaACO (A), CaACS (B) and CaETR (C) isoforms of Green and Breaker treated capsicum at 0 DAT. C, Control (open bars); E, 100 μL/L ethylene treatment for 24 h (striped bars); M, 500 nL/L 1-MCP treatment for 24 h (closed bars). Significantly different levels were determined using Least Significant Difference (P < 0.05) of 1-way ANOVA at each stage (Green and Breaker, respectively) and are indicated by different letters on bars (±SE of n = 3 biological replicates). Gene expression was normalised relative to CaGAPdH expression according to the Methods. Note that the relative expression axis was set according to respective isoforms.