Table 1 Number of selected lines and plants, PCR positive plants and transformation efficiency of cultivar Wanad transformed with (Pina), (Pinb) silencing cassettes and control pMC161 (pMCG) vector
Silencing cassette | Number of explants | Number of selected | PCR positive plants / transformation efficiency [%]a | |
|---|---|---|---|---|
| Â | Â | Lines (SD) | Plants (SD) | Â |
(Pina) | 582 | 17 ± 0.70 | 39 ± 4.94 | 14 / 2.4 |
(Pinb) | 1460 | 3 ± 1.22 | 17 ± 6.94 | 2 / 0.14 |
(Pina)+(Pinb) | 1960 | 0 | 0 | 0 / 0.00 |
(pMCG) | 452 | 8 ± 1.92 | 14 ± 3.41 | 4 / 0.88 |
Sum /mean | 4454 | 28 ± 1.28 | 70 ± 5.1 | 20 / 1.14 |