Figure 2

Genome-wide genotyping with CHR markers. (a) Multiplex PCR products for 40 Col-0-specific dominant markers were size-separated by agarose-gel electrophoresis and stained with ethidium bromide. Sizes in basepairs (bp) for the DNA ladder (leftmost lane) are at left. Marker DNA was PCR-amplified from the Col-0 and Ty-0 F₁ hybrid (C/T), accession Ty-0 (T/T) and C-T BC₁ hybrids, 5A2, 5A3, 5A4, 5A6 and 5A7. Lines to the right indicate the expected positions of marker bands. Markers are named CHRx.n, where x is the chromosome and n is the relative position on the chromosome. (b) Genotypes of markers in five BC₁ hybrids are from banding phenotypes in (a). Markers are ordered with respect to their position on chromosomes. Genotype C/T (C) is shown with black on white type, and genotype T/T (T) is white on black type.