Figure 8

SUB::SUB:EGFP expression in five-days-old root tips of WT and doq-1 mutants in the absence and presence of BFA. Confocal micrographs are shown. Two independent lines transgenic for a functional SUB:SUB:EGFP reporter [6] exhibiting a GFP-based signal were analyzed for each genotype. GFP-based signal is shown in (A, D, G, J), FM4-64-based counterstain in (B, E, H, K) and the overlay in (C, F, I, L). Mid-optical section through a wild-type root tip highlights the plasma membrane localization of SUB:EGFP (A-C). Tangential optical section through the epidermis of a wild-type root tip treated with 50 μm BFA for 30 minutes reveals the typical dotted structures that indicate the presence of BFA compartments (D-F). A similar set is shown for untreated doq-1 roots (G-I) and BFA-treated doq-1 roots (J-L). Scale bars: 10 μm.